Objectives: Triphala Churna (TC) is a tridoshashamak Ayurvedic rasayana used for treating various diseases such as skin diseases, diabetes, and as a mild laxative. Methods: In this study, TC was prepared and quality standardized standardized using various parameters such as macroscopical evaluation, physical properties, physicochemical properties, and phytochemical analysis (HPTLC fingerprinting and quantification) methods to assess its quality and purity. Results: The qualitative and quantitative phytochemical assays via HPTLC revealed concentrations of Gallic acid as 3.62 ± 0.05%, 3.67 ± 0.04%, 1.52 ± 0.03%, and 2.96 ± 0.02% for Amlaki (Emblica officinalis), Haritiki (Terminalia chebula), Vibhitki (Terminalia bellerica), and TC, respectively. Conclusion: This study provides valuable insights into establishing robust quality control measures and developing reliable assays for both Triphala Churna (TC) and its individual components.