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2508001131
  • Open Access
  • Review

Cell Electrospinning: Electrohydrodynamic Effects on Cell Viability and Beyond

  • Anne-Kathrine Kure Larsen,   
  • Menglin Chen *

Received: 09 Jun 2025 | Revised: 09 Aug 2025 | Accepted: 13 Aug 2025 | Published: 19 Aug 2025

Abstract

Electrospinning is a widely used technique for creating nano- to microscale fibers that resembles the fibrous structure of the extracellular matrix (ECM) environment, crucial for tissue engineering and disease modelling. Directly incorporating living cells into the electrospinning process, ‘cell electrospinning’, has evolved in the last two decades as a new biofabrication method combining homogenous cell loading with the potential of single cell resolution. However, keeping cells viable and functional during the electrohydrodynamic process is an ongoing challenge. In this review, key parameters in electrospinning affecting mammalian cell viability and functionality are assessed with the goal of identifying the most critical ones in the successful production of living cell-embedded fibers. The review further outlooks the potential mechanobiological and electrophysiological effects on the cells exposed under the electrohydrodynamic condition to layout a couple unexplored applications.

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DOI
10.53941/mi.2025.100025
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Volume 2, Issue 3
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Larsen, A.-K. K.; Chen, M. Cell Electrospinning: Electrohydrodynamic Effects on Cell Viability and Beyond. Materials and Interfaces 2025, 2 (3), 332–347. https://doi.org/10.53941/mi.2025.100025.
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