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Controlled Codelivery of Hydrogen Sulfide and Nitric Oxide for the Treatment of Full-Thickness Skin Wound

  • Meng Qian 1, 2, †,   
  • Guangbo Ji 2, †,   
  • Pei Liu 2,   
  • Yating Zhang  2,   
  • Weiliang Deng  2,   
  • Zhixin Xu 2,   
  • Huan Wang 1,   
  • Jiansong Cheng 3,   
  • Qiang Zhao 1, 2, *

Received: 28 Jul 2025 | Revised: 15 Aug 2025 | Accepted: 17 Sep 2025 | Published: 19 Sep 2025

Abstract

Chronic wounds present significant clinical challenges stemming from persistent inflammation, impaired angiogenesis, and disrupted tissue homeostasis. Nitric oxide (NO) and hydrogen sulfide (H2S) are important gaseous signalling molecules that play critical regulatory roles in the pathophysiology of numerous diseases. In the present study, we designed a novel prodrug, TSNO, which releases H2S and NO in a controlled manner under the catalysis of GSH and the engineered enzyme A4-β-GalH363A. Furthermore, a functional wound dressing was prepared by incorporating TSNO into an electrospun poly(ε-caprolactone) (PCL) mat, and the therapeutic efficacy was evaluated in a full-thickness skin wound model established in mice. Results showed that the functional wound dressing effectively accelerated the wound healing process by enhancing collagen synthesis, promoting angiogenesis, and regulating inflammation via controlled codelivery of H2S and NO. Taken together, the functional wound dressing developed in this study represents a promising candidate for treating large-scale skin wounds in the clinic.

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Supplementary Materials
DOI
10.53941/rmd.2025.100013
Issue
Volume 2, Issue 3
How to Cite
Qian, M.; Ji, G.; Liu, P.; Zhang , Y.; Deng , W.; Xu, Z.; Wang, H.; Cheng, J.; Zhao, Q. Controlled Codelivery of Hydrogen Sulfide and Nitric Oxide for the Treatment of Full-Thickness Skin Wound. Regenerative Medicine and Dentistry 2025, 2 (3), 13. https://doi.org/10.53941/rmd.2025.100013.
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