2509001631
  • Open Access
  • Article

Invigoration of Expired Soybean Seeds Using Plant Extract: Effects on Viability and Growth

  • Yaya Hasanah *,   
  • Faiyaz Titan Hasibuan

Received: 13 May 2025 | Revised: 29 Sep 2025 | Accepted: 30 Sep 2025 | Published: 10 Oct 2025

Abstract

Expired seeds exhibit reduced viability and are difficult to germinate; however, appropriate invigoration can partially restore performance. We evaluated the effects of plant extracts on the viability and growth of expired soybean seeds using a factorial randomized complete block design with two factors and three replicates. Factor 1 comprised extract type—distilled water (control), young coconut water (25%), bamboo shoot extract (25%), and mung bean sprout extract (25%). Factor 2 was immersion duration: 6, 12, and 18 h. Across outcomes, 25% bamboo shoot extract produced significantly higher vigor index, maximum growth potential, and pollen fertility than the control, 25% coconut water, or 25% sprout extract. A 6-h immersion maximized vigor index, maximum growth potential, plant height at 5–6 weeks after planting, and pollen fertility compared to 12 or 18 h. The combination of 25% bamboo shoot extract and 6-h immersion accelerated the onset of flowering.

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How to Cite
Hasanah, Y.; Hasibuan, F. T. Invigoration of Expired Soybean Seeds Using Plant Extract: Effects on Viability and Growth. Annals of Agri-bio Research 2025, 30 (2), 107–113. https://doi.org/10.53941/agrbio.2025.1000029.
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