Scilight Press

The potential of Trichoderma reseei and isolate Aspergillus flavus OR for production of cellulase enzyme was checked by using rice straw as the lignocellulosic substrate. A maximum cellulase yield of 0.054 IU/ml at 120 h incubation was achieved using the T. reesei. Further incubation decreased the cellulase activities which proved that incubation period played a major role in the enzyme production process and should be monitored very carefully. A. flavus OR gave the highest cellulase activity of 0.042 IU/ml at 192 h. Based on the results of the hydrolytic enzymatic activity, both the microorganisms were seen as an efficient cellulose degrader and could replace the commercial enzymes which are not cost-effective. Rice straw also acted as a promising feed stock in the production of energy. The bioconversion of lignocellulosic biomass received global attention in the past few decades and is now a subject of intensive research in the field of developing liquid fuels such as bioethanol.