Scilight Press

Cauliflower is an important Cole crop grown in India but the attack of insects-pests hampers the production of the crop. Generation of the transgenic crop resistant to insects can serve to be a solution for the problem. Therefore, establishment of an optimized in vitro regeneration protocol of the Indian cauliflower is a prerequisite, hence, two explants, namely, cotyledon and hypocotyls were obtained from the seedlings of the Indian cauliflower variety Pusa Snowball K-1. Results showed that the shoot regeneration from hypocotyls explants on media containing 1 mg/l BAP along with the combination 0.1 mg/l IAA was best suited and for root regeneration 1.5 mg/l IBA was found to be most effective. Further, standardized protocol complemented for Agrobacterium-mediated transformation against the insect Diamondback moth i.e. the DBM.