To evaluate the effects of nano-copper on pathogenic bacteria in vivo, 400 fertilized eggs of quail were used; divided into four groups (T1, T2, T3 and T4) each group of 100 eggs was injected with E. coli bacteria (1 m × 10-7). Further, groups T2, T3 and T4 were injected with copper nanoparticles at concentrations of 14, 16 and 18 ppm, respectively. Each group was exposed to 38 ℃ in hatcher to receive 30 chicks from the hatched eggs. Significant increase was observed in T1 for the percentage of embryo mortality and total mortality, in group T4 for the rate of weight gain and feed conversion efficiency, and in group T3 for the percentage of hatching, chick weight at hatching and total live body weight. The rate at which feed was consumed was also significantly higher in T4.



