Scilight Press

Nine bread wheat genotypes (HD 2851, HD 2967, HD 3086, PBW 502, PBW 550, PBW 677, UNNAT 343, WH 1105 and W H711) were pollinated with pollen of the maize genotype (PRMH 531) followed by 100 ppm 2,4-D application for haploid embryo induction. Data were collected for the number of emasculated florets, number of pollinated florets, number of pseudo seeds formed, pseudo seed formation frequency, pseudo-seed and haploid embryo formation efficiency. Three treatments comprising single dose (after 24 h), 2 doses (after 24 and 48 h) and 3 doses (after 24, 48 and 72 h) were used. The results revealed that the application of 2, 4-D at 100 ppm three times, i.e. 24, 48, and 72 h, resulted in a significantly high frequency of pseudo-seeds and haploid embryos when crossed with PRMH 351.