Euchresta horsfieldii (Lesch.) is one of the medicinal plants having many benefits and in high demand. This study was aimed at obtaining the proper concentrations of BAP and 2,4-D for increasing the production of psychic in vitro. The study used a completely randomized design (CRD) which was arranged in a factorial design consisting of two treatment factors with 25 treatment combinations repeated three times. The addition of 2 ppm BAP and 2,4-D 0 ppm showed the best response on shoot height, number of shoots and number of leaves. Most of the emerging calluses were green with a compact callus texture. Callus appeared in all treatments, while shoots appeared mostly on media with a concentration of 2,4- D 0 ppm. Further research can be done by using embryos as explants, using different growth regulators.



