JMNP/
Articles/
2504000551
  • Open Access
  • Article
Drug Standardization, HPTLC Finger Printing, Toxicity Research Studies of ASU Herbaceous Plant Fruit Seeds Part Samples of Ipomoea nil (Linn.) Roth
  • Pawan Kumar Sagar  1, *,   
  • Suryansh Kashyap 1,   
  • Mageswari S. 2,   
  • P. Meera Devi Sri 2,   
  • Asma Sattar Khan 1

Received: 21 Nov 2024 | Revised: 27 Feb 2025 | Accepted: 18 Mar 2025 | Published: 22 Apr 2025

Abstract

This study aims to evaluate the drug standardization research, physico-chemical, toxicity research studies of the fruit seeds part of plant of I N. drug standardization research, physico-chemical, toxicity research studies of ASU (Ayurveda, Siddha, Unani) herbal products remains a big challenging task. There needs to be more than the advance investigation research studies and screening parameters to validation, authenticate and differentiate adulterants in Ipomoea nil (Linn.) Roth medicinal plant is one of the herb used to treat various health wellness and therapeutic illness of public mankind. Plant samples of I N. powder were carried out using standard methods. The Quality, safety and toxicity effects of the tested drug samples were also investigated. estimated and investigated research studies data’s of I N. have shown that all the parameters were within the AYUSH/WHO permissible limits. The tested drug samples showed significant Quality, safety and toxicity studies against certain pathogens organisms and promising anti-pathogenic activity. In the investigated studies of DSR, HPTLC finger printing investigation, QC, Toxicity research findings revealed that the revalidated test drug samples was free from adulterations. This investigated herb research data confirmed to drug standardization and therapeutically may treat that the drug is safe for internal use and cures in Antioxidant, Anti-Inflammatory, Antimicrobial, Antispasmodic, Anticancer, Anti-tumor, Antiproliferative Activities, Multidrug-Resistance Efflux-Inhibiting Activity, bronchodilatory along with Antiasthmatic potential, Diuretic, anthelminthic and deobstruant and are prescribed for dropsy and constipation, Antihypertensive and cardio-protective effects, Investigated drug had classically, traditional and alternative ASU medicine I N. used as a Cold, Dropsy, Gout, Joint pain, Vitiligo, Itching, Asthma and Anthelmintic.

References 

  • 1.
    Sagar, P.K.; Sajwan, S.; Murugeswaran, R. Bauhinia Spp.,- Kachnar: Medicinal plant biodiversity, geographical distribution, bioactive phytochemical constituents and their ethno pharmacology, therapeutics medicinal values. Asian J. Pharma. Res. Dev. 2025, 13, 55–65.
  • 2.
    Sagar, P.K.; Sajwan, S.; Sri, P.M.D.; et al. Scientific DSR validation, pharmacognostical, biodiversity, toxicological research studies of Nyctanthes arbortristis L.- aerial leaves part and their pharmacological, therapeutic medicinal values. Int. J. Pharma. Sci. 2025, 3, 1641–669.
  • 3.
    Sagar, P.K.; Sajwan, S.; Mageswari, S.; et al. Scientific assessment, research studies of botanical, pharmacognostical,bio diversity and toxicological of ASU herbal drug Kaladana / Habb-ul-Neel (Ipomoea nil (Linn.) Roth.) seeds. J. Pharmacogn. Phytochem. 2024, 13, 112–123. https://doi.org/10.22271/phyto.2024.v13.i6b.15171.
  • 4.
    Sagar, P.K.; Ahmed, M.W.; Kashyap, S.; et al. Drug standardization, HPTLC finger printing, toxicological research studies of ASU herbal drug fruit seeds part of Peucedanum grande C.B. Clarke. Int. J. Herb. Med. 2024, 12, 15–23. https://doi.org/10.22271/flora.2024.v12.i6a.954.
  • 5.
    Sagar, P.K.; Mageswari, S.; Ahmed, M.W. An conscious review validation biodiversity, pharmacognostical, pharmacological, toxicological research studies and ther apeuticpotential of ASU herbal drugs-Seed part of Baphali /Duku/ Duqu (Peucedanum grande C.B. Clarke). J. Pharmacogn. Phytochem. 2024, 13, 396–404. https://doi.org/10.22271/phyto.2024.v13.i4e.15030.
  • 6.
    Sagar, P.K.; Kashyap, S.; Kurele, R.; et al. Quality assurance, shelf life, stability studies of ASU herbal, medicinal plants - Syzygium aromaticum (Linn.) Merr LM Perry and W. fruticosa (Linn.) Kurz. J. Pharmacogn. Phytochem. 2024, 13, 323–333. https://doi.org/10.22271/phyto.2024.v13.i4d.15025.
  • 7.
    Sagar, P.K.; Kashyap, S.; Kurele, R.; et al. Scientific standardization, quality control, antimicrobial potential, toxicology studies having effective therapeutic uses of (Woodfordia Fruticosa (Linn.) kurz) flowers part. World J. Pharm. Pharm. Sci. 2024, 13, 1301–1323.
  • 8.
    Sagar, P.K.; Ahmed, N.Z.; Khan, A.S.; et al. Pharmacopeial standard development, pharmacognosy, HPTLC. fingerprinting and physicochemical research studies of Beninca Hispida (Thunb.). Int. J. Recent Sci. Res. 2024, 15, 4644–4651.
  • 9.
    Sagar, P.K.; Khan, A.S.; Sajwan, S.; et al. Anconcise over view on standardization research of ASU-TAM herbal formulated and single drugs, products. Int. J. Pharm. Pharm. Sci. 2024, 6, 86–95. https://doi.org/10.33545/26647222.2024.v6.i1b.91.
  • 10.
    World Health Organization. Quality Control Methods for Medicinal Plant Materials (1998). Available online: https://www.who.int/docs/default-source/medicines/norms-and-standards/guidelines/qualitycontrol/quality-control-methods-for-medicinal-plant-materials.pdf?sfvrsn=b451e7c6_0 (accessed on 1 July 2024).
  • 11.
    Alqahtani, T.; Parveen, S.; Alghazwani, Y.; et al. Pharmacological validation for the folklore use of Ipomoea nil against asthma: In-vivo and in-vitro evaluation. Molecules 2022, 27, 4653. https://doi.org/10.3390/molecules27144653.
  • 12.
    Ministry of Health and Family Welfare, Government of India. The Ayurvedic Pharmacopoeia of India. Available online: https://www.ayurveda.hu/api/API-Vol-1.pdf (accessed on 1 July 2024)
  • 13.
    Chaudhry, M.A.; Mushtaq, M.N.; Bukhari, I.A.; et al. Ipomoea hederacea Jacq.: A plant with promising anti-hypertensive and cardio-protective effects. J. Ethanopharmacol. 2021, 268, 113584.
  • 14.
    Ipomoea nil (L.) Rath. Available online: https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:1071575-2 (accessed on 1 January 2020).
  • 15.
    Khare, C.P. Ipomoea nil (Linn.), Roth; In Indian Medicinal Plant; Springer: New York, NY, USA, 2014, pp. 1. https://doi.org/10.1007/978-0-387-70638-2_800.
  • 16.
    Kirtikar, K.; Basu, B. Indian Medicinal Plants; International Book Distributors: New Delhi, India, 1987; vol. 1.
  • 17.
    Satyavati, G.V.; Raina, M.K.; Sharma, M. Medicinal Plants of India, In Indian Council of Medical Research; New Delhi Indian Council of Medical Research: New Delhi, India, 1987; vol. 2, pp. 398–399.
  • 18.
    Kadam, D.K.; Ahire, P.D.; Bhoye, J.V.; et al. Comparative standardization study of three Triphala churna formulation. Int. J. Pharmacog. 2018, 4, 71–78.
  • 19.
    Parihar, A.K.S.; Sahu, U.; Karbhal, K.S.; et al. Quality Control of Triphala Churna. J. Med. Nat. Prod. 2024, 1,100003. https://doi.org/10.53941/jmnp.2024.100003.
  • 20.
    Singh, D.P.; Govindarajan, R.; Rawat, A.K.S. High-performance liquid chromatography as a tool for the chemical standardization of Triphala- An Ayurvedic formulation. Phytochem. Anal. 2008, 19, 164–168. https://doi.org/10.1002/pca.1032.
  • 21.
    Jain, V.; Saraf, S.; Saraf, S. Standardization of Triphala churna: Spectrophotometric approach. Asian J. Chem. 2007, 19, 1406.
  • 22.
    Akinniyi, G.; Jeonghee Lee, J.; Kim, H.; et al. A medicinal halophyte Ipomoea pescaprae (Linn.) R.Br.: A review of its botany, traditional uses, phytochemistry, and bioactivity. Mar. Drugs 2022, 20, 329. https://doi.org/10.3390/md20050329.
  • 23.
    Govaerts, R.; Nic Lughadha, E.; Black, N.; et al. The World Checklist of Vascular Plants, a continuously updated resource for exploring global plant diversity. Sci. Data 2021, 8, 215. https://doi.org/10.1038/s41597-021-00997-6.
  • 24.
    Sagar, P.K.; Ahmed, N.Z. DSR, physicochemical HPTLC, GC-MS and anti-bacterial potential, toxicology studies of achloroform and ethanolic extracts of Tagetes Erecta L. flowers part. World J. Pharm. Pharm. Sci. 2024, 13, 1278–1300. https://doi.org/10.20959/wjpps20246-27457.
  • 25.
    Sagar, P.K.; Khan, A.S.; Sajwan, S.; et al. Scientific standardization, quality control screening study on effective therapeutic uses of Dhataki/ DhaiPhool/ Gul-e-Dhawa (Woodfordia fruticose (Linn.) Kurz). Int. J. Appl. Res. 2024, 10, 39–53. https://doi.org/10.22271/allresearch.2024.v10.i5a.11729.
  • 26.
    Sagar, P.K.; Khan, A.S. Pharmacognosy, physicochemistry, toxicity and quality development study Gul-e-Saad Barg (Marigold)-Tagetes erecta L. flower of plant. Int. J. Appl. Res. 2024, 10, 54–66. https://doi.org/10.22271/allresearch.2024.v10.i5a.11730.
  • 27.
    Sajwan, S.; Negi, R.; Sagar, P.K. Pharmacognostic, physicochemical and therapeutic potential of Woodfordia Fruticosa (L.) Kurz. Adv. Int. J. Multidiscip. Res. 2024, 2, 1–5.
  • 28.
    Sagar, P.K.; Ahmed, N.Z.; Khan, A.S.; et al. Pharmacopeial standard development, HPTLC finger printing and physicochemical, research studies of polyherbal classical drug Raughan-E-Mukhtara. J. Sci. Res. Chem. 2023, 8, 38–52.
  • 29.
    Sagar, P.K.; Khan, A.S.; Meena, R.P.; et al. Scientific standard validation and HPTLC fingerprinting studies of polyherbal unani formulation Majoon-e-Maddat-ul-Hayat Jadwari, J. Sci. Res. Chem. 2023, 8, 14–29.
  • 30.
    Sagar, P.K.; Khan, A.S.; Sonali, S. Pharmacognosy, physicochemical, toxicity quality evolution studies of Marigold-Tagetes erecta L. flower of plant. Int. J. Pharm. Pharm. Sci. 2022, 4, 57–66. http://doi.org/10.33545/26647222.2022.v4.i2a.90.
  • 31.
    Kumar, J.; Sagar, P.K.; Sajwan, S.; et al. Pharmacpgnostical and HPTLC fingerprinting physcico-chemical studies of single drug Ban Ajwan or Hasha (Thymus Serpyllum L.) (WholePlant). Int. J. Multidiscip. Educ. Res. 2021, 10, 1.
  • 32.
    Ministry of AYUSH, Govt. of India. Habb-ul- Neel (Seed); In The Unani Pharmacopeia of India; PCIM&H: Ghaziabad, India, 2007; Part I, Volume II, pp. 47–48.
  • 33.
    WHO global atlas of traditional, complementary and alternative medicine. Available online: https://www.who.int/publications/i/item/9241562862 (accessed on 1 January 2025)
  • 34.
    EMEA. Quality of herbal medicinal products/traditional herbal medicinal products - Scientific guideline. Available online: https://www.ema.europa.eu/en/quality-herbal-medicinal-products-traditional-herbal-medicinal-products-scientific-guideline (accessed on 1 January 2025)
  • 35.
    Official Methods of Analysis of AOAC International, 18thed.; Horwitz, W., Latimer, G.W.; Eds.; AOAC International: Rockville, MD, USA, 2005; Chapter 3, pp. 10–11; Chapter 10, pp. 18–23; Chapter 26, p.17.
  • 36.
    WHO. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine (2000). Available online: https://www.who.int/publications/i/item/9789241506090 (accessed on 1 January 2025)
  • 37.
    Sethi, P.D. High Performance Thin Layer Chromatography, 1st ed.; CBS Publishers and Distributers: New Delhi, India, 1996; Volume X; pp. 1–56.
  • 38.
    Wagner, H.; Biadi, S. Plant Drug Analysis—A Thin Layer Chromatography Atlas, 2nd ed.; Springer: Berlin/ Heidelberg, Germany, 1996.
  • 39.
    Sharma, S.K.; Alam, A. Identification of secondary metabolites, antioxidantactivity, and GC-MS analysis of Launaea procumbens (Roxb.) Amin (Angiosperm: A steraceae): Aneglected plant with therapeutic value. J. Nat. Resour. Hum. Health 2023, 3, 462–468. http://doi.org/10.53365/nrfhh/174619.
Share this article:
Download PDF
DOI
10.53941/jmnp.2025.100008
Issue
Volume 2, Issue 2
How to Cite
Sagar , P. K.; Kashyap, S.; Mageswari S.; Devi Sri, P. M.; Khan, A. S. Drug Standardization, HPTLC Finger Printing, Toxicity Research Studies of ASU Herbaceous Plant Fruit Seeds Part Samples of Ipomoea nil (Linn.) Roth. Journal of Medicinal Natural Products 2025, 2 (2), 100008. https://doi.org/10.53941/jmnp.2025.100008.
RIS
BibTex
Copyright & License
article copyright Image
Copyright (c) 2025 by the authors.