This study aims to evaluate the drug standardization research, physico-chemical, toxicity research studies of the fruit seeds part of plant of I N. drug standardization research, physico-chemical, toxicity research studies of ASU (Ayurveda, Siddha, Unani) herbal products remains a big challenging task. There needs to be more than the advance investigation research studies and screening parameters to validation, authenticate and differentiate adulterants in Ipomoea nil (Linn.) Roth medicinal plant is one of the herb used to treat various health wellness and therapeutic illness of public mankind. Plant samples of I N. powder were carried out using standard methods. The Quality, safety and toxicity effects of the tested drug samples were also investigated. estimated and investigated research studies data’s of I N. have shown that all the parameters were within the AYUSH/WHO permissible limits. The tested drug samples showed significant Quality, safety and toxicity studies against certain pathogens organisms and promising anti-pathogenic activity. In the investigated studies of DSR, HPTLC finger printing investigation, QC, Toxicity research findings revealed that the revalidated test drug samples was free from adulterations. This investigated herb research data confirmed to drug standardization and therapeutically may treat that the drug is safe for internal use and cures in Antioxidant, Anti-Inflammatory, Antimicrobial, Antispasmodic, Anticancer, Anti-tumor, Antiproliferative Activities, Multidrug-Resistance Efflux-Inhibiting Activity, bronchodilatory along with Antiasthmatic potential, Diuretic, anthelminthic and deobstruant and are prescribed for dropsy and constipation, Antihypertensive and cardio-protective effects, Investigated drug had classically, traditional and alternative ASU medicine I N. used as a Cold, Dropsy, Gout, Joint pain, Vitiligo, Itching, Asthma and Anthelmintic.