The parameters such as temperature and pH have an imperative impact on the sporophore expansion of mushroom and may turn out to be as a limiting factor in cultivation. The present study was aimed at investigating the optimal growth parameters for the mycelia of the Hericium erinaceus (HE 203) strain under submerged conditions. The optimum temperature for mycelial growth was found to be 24°C, although growth occurred in the broader temperature range of 20 to 28°C. In case of pH, the range lied in between 5-8 but ideal pH for maximum mycelial development was at pH 6. Further under ex-vivo conditions, the optimized inoculum showed maximum yield on saw dust as compared to wheat straw.



