Scilight Press

Toxoplasma gondii is considered an obligatory intracellular parasite that causes toxoplasmosis. This study included 100 infertile women. Serum and whole blood were taken from the included subjects. The DNA was extracted from the whole blood by using a commercial kit. Real-time PCR was done for all the samples to detect the presence of T. gondii. PCR was done for the positive samples to amplify a target sequence within the rop-17 gene. The PCR product was sequenced to reveal the variations within the rop-17 gene. The ELISA test was done to measure the serum level of SOD and GPX. The real time-PCR showed 43% frequency of the samples. The conventional PCR was used to amplify the rop-17 gene in the positive samples but showed a successful amplification in 92% of the real-time PCR positive samples. The results of the sequences showed different variations within the rop-17 gene. GPX and SOD were both higher in patients (93.76±9.6 and 89.6±9.4, respectively) than in control (76.5±31.4 and 85.6±7.43, respectively).