2509001633
  • Open Access
  • Article

Severe Mutations on Open Reading Frame (ORF5) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)2 in Korean Pig Farm

  • HeeJoon Kim 1,   
  • Woohyeok Han 1,   
  • Myungdal Yoon 2,   
  • Seungheon Lee 2,   
  • Donghwan Song 3,   
  • Jihyung Hwang 3,   
  • Mi-Jeong Choi 3,4,   
  • Sinae Kim 5,*

Received: 04 Aug 2025 | Revised: 08 Sep 2025 | Accepted: 29 Sep 2025 | Published: 30 Sep 2025

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV)2 is a highly contagious Ribonucleic acid (RNA) virus of the Arteriviridae family that causes reproductive and respiratory disorders in pigs, posing a major economic threat to the swine industry in Republic of Korea and worldwide. Recently, a PRRSV2-related virus was isolated from a pig farm in Gyeonggi-do, Republic of Korea, and its open reading frame 5 (ORF5, also known as GP5; glycoprotein 5) was sequenced to characterize the strain. Sequence alignment showed only 57.7% identity to PRRSV1 references, consistent with PRRSV2 rather than PRRSV1; however, surprisingly, due to the severe mutation in ORF5, this sequence does not belong to any known PRRSV2 strain with an average identity of 87.4%. These findings raise concerns about the effectiveness of currently available PRRSV2 vaccines in Republic of Korea and suggest that vaccines derived from newly isolated strains may be required for more effective protection.

References 

  • 1.
    Benfield, D.A.; Nelson, E.; Collins, J.E.; Harris, L.; Gaukler, S.; Robison, D.; Christianson, W.T.; Morrison, R.B.; Gorcyca, D.; Chladek, D. et al. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Investig. 1992, 4, 127–133.
  • 2.
    Cavanagh, D. Nidovirales: A new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 1997, 142, 629–633.
  • 3.
    Pesch, S.; Meyer, C.; Ohlinger, V.F. New insights into the genetic diversity of European porcine reproductive and respiratory syndrome virus (PRRSV). Vet. Microbiol. 2005, 107, 31–48.
  • 4.
    Forsberg, R.; Storgaard, T.; Nielsen, H.S.; Oleksiewicz, M.B.; Cordioli, P.; Sala, G.; Hein, J.; Bøtner, A. The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe. Virology 2002, 299, 38–47.
  • 5.
    Minskaia, E.; Hertzig, T.; Gorbalenya, A.E.; Campanacci, V.; Cambillau, C.; Canard, B.; Ziebuhr, J. Discovery of an RNA virus 3′ → 5′ exoribonuclease that is critically involved in coronavirus RNA synthesis. Proc. Natl. Acad. Sci. USA 2006, 103, 5108–5113.
  • 6.
    Nga, P.T.; Parquet Mdel, C.; Lauber, C.; Morita, K.; Snijder, E.J.; Gorbalenya, A.E.; Ooi, E.E.; Baker, S.C. Discovery of the first insect nidovirus, a missing evolutionary link in the emergence of the largest RNA virus genomes. PLoS Pathog. 2011, 7, e1002215.
  • 7.
    Shi, M.; Lam, T.T.; Hon, C.C.; Murtaugh, M.P.; Davies, P.R.; Hui, R.K.; Li, J.; Wong, L.T.; Yip, C.W.; Jiang, J.W.; et al. Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J. Virol. 2010, 84, 8700–8711.
  • 8.
    Garner, M.G.; Whan, I.F.; Gard, G.P.; Phillips, D. The expected economic impact of selected exotic diseases on the pig industry of Australia. Rev. Sci. Tech. 2001, 20, 671–685.
  • 9.
    Holtkamp, D.; Kliebenstein, J.; Neumann, E.; Zimmerman, J.; Rotto, H.; Yoder, T.; Wang, C.; Yeske, P.; Mowrer, C.; Haley, C. Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers. J. Swine Health Prod. 2013, 21, 78–84.
  • 10.
    Neumann, E.J.; Kliebenstein, J.B.; Johnson, C.D.; Mabry, J.W.; Bush, E.J.; Seitzinger, A.H.; Green, A.L.; Zimmerman, J.J. Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J. Am. Vet. Med. Assoc. 2005, 227, 385–392.
  • 11.
    Nieuwenhuis, N.; Duinhof, T.F.; van Nes, A. Economic analysis of outbreaks of porcine reproductive and respiratory syndrome virus in nine sow herds. Vet. Rec. 2012, 170, 225.
  • 12.
    Batista, L.; Pijoan, C.; Dee, S.; Olin, M.; Molitor, T.; Joo, H.S.; Xiao, Z.; Murtaugh, M. Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts. Can. J. Vet. Res. 2004, 68, 267–273.
  • 13.
    Done, S.H.; Paton, D.J.; White, M.E. Porcine reproductive and respiratory syndrome (PRRS): A review, with emphasis on pathological, virological and diagnostic aspects. Br. Vet. J. 1996, 152, 153–174.
  • 14.
    Halbur, P.G.; Paul, P.S.; Frey, M.L.; Landgraf, J.; Eernisse, K.; Meng, X.J.; Lum, M.A.; Andrews, J.J.; Rathje, J.A. Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet. Pathol. 1995, 32, 648–660.
  • 15.
    Halbur, P.G.; Paul, P.S.; Meng, X.J.; Lum, M.A.; Andrews, J.J.; Rathje, J.A. Comparative pathogenicity of nine US porcine reproductive and respiratory syndrome virus (PRRSV) isolates in a five-week-old cesarean-derived, colostrum-deprived pig model. J. Vet. Diagn. Investig. 1996, 8, 11–20.
  • 16.
    Frossard, J.P.; Hughes, G.J.; Westcott, D.G.; Naidu, B.; Williamson, S.; Woodger, N.G.; Steinbach, F.; Drew, T.W. Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates. Vet. Microbiol. 2013, 162, 507–518.
  • 17.
    Stadejek, T.; Stankevicius, A.; Murtaugh, M.P.; Oleksiewicz, M.B. Molecular evolution of PRRSV in Europe: Current state of play. Vet. Microbiol. 2013, 165, 21–28.
  • 18.
    Li, Y.; Zhou, L.; Zhang, J.; Ge, X.; Zhou, R.; Zheng, H.; Geng, G.; Guo, X.; Yang, H. Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China. PLoS Pathog. 2014, 10, e1004216.
  • 19.
    García-Nicolás, O.; Baumann, A.; Vielle, N.J.; Gómez-Laguna, J.; Quereda, J.J.; Pallarés, F.J.; Ramis, G.; Carrasco, L.; Summerfield, A. Virulence and genotype-associated infectivity of interferon-treated macrophages by porcine reproductive and respiratory syndrome viruses. Virus Res. 2014, 179, 204–211.
  • 20.
    Karniychuk, U.U.; Geldhof, M.; Vanhee, M.; Van Doorsselaere, J.; Saveleva, T.A.; Nauwynck, H.J. Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate. BMC Vet. Res. 2010, 6, 30.
  • 21.
    Morgan, S.B.; Frossard, J.P.; Pallares, F.J.; Gough, J.; Stadejek, T.; Graham, S.P.; Steinbach, F.; Drew, T.W.; Salguero, F.J. Pathology and Virus Distribution in the Lung and Lymphoid Tissues of Pigs Experimentally Inoculated with Three Distinct Type 1 PRRS Virus Isolates of Varying Pathogenicity. Transbound. Emerg. Dis. 2016, 63, 285–295.
  • 22.
    Morgan, S.B.; Graham, S.P.; Salguero, F.J.; Cordón, P.S.; Mokhtar, H.; Rebel, J.M.; Weesendorp, E.; Bodman-Smith, K.B.; Steinbach, F.; Frossard, J.P. Increased pathogenicity of European porcine reproductive and respiratory syndrome virus is associated with enhanced adaptive responses and viral clearance. Vet. Microbiol. 2013, 163, 13–22.
  • 23.
    Weesendorp, E.; Morgan, S.; Stockhofe-Zurwieden, N.; Popma-De Graaf, D.J.; Graham, S.P.; Rebel, J.M. Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence. Vet. Microbiol. 2013, 163, 1–12.
  • 24.
    Pamornchainavakul, N.; Paploski, I.A.; Makau, D.N.; Baker, J.P.; Huang, J.; Ferreira, C.P.; Corzo, C.A.; Rovira, A.; Cheeran, M.C.; Lycett, S.; et al. Experimental evidence of vaccine-driven evolution of respiratory syndrome virus type 2. Virus Evol. 2025, 11, veaf056.
  • 25.
    Sun, Q.; Xu, H.; An, T.; Cai, X.; Tian, Z.; Zhang, H. Recent Progress in Studies of Porcine Reproductive and Respiratory Syndrome Virus 1 in China. Viruses 2023, 15, 1528.
  • 26.
    Lowe, J.F.; Husmann, R.; Firkins, L.D.; Zuckermann, F.A.; Goldberg, T.L. Correlation of cell-mediated immunity against porcine reproductive and respiratory syndrome virus with protection against reproductive failure in sows during outbreaks of porcine reproductive and respiratory syndrome in commercial herds. J. Am. Vet. Med. Assoc. 2005, 226, 1707–1711.
  • 27.
    Zuckermann, F.A.; Garcia, E.A.; Luque, I.D.; Christopher-Hennings, J.; Doster, A.; Brito, M.; Osorio, F. Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge. Vet. Microbiol. 2007, 123, 69–85.
  • 28.
    Kim, S.; Lee, J.H.; Lee, S.; Shim, S.; Nguyen, T.T.; Hwang, J.; Kim, H.; Choi, Y.O.; Hong, J.; Bae, S.; et al. The Progression of SARS Coronavirus 2 (SARS-CoV2): Mutation in the Receptor Binding Domain of Spike Gene. Immune Netw. 2020, 20, e41.
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Kim, H.; Han, W.; Yoon, M.; Lee, S.; Song, D.; Hwang, J.; Choi, M.-J.; Kim, S. Severe Mutations on Open Reading Frame (ORF5) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)2 in Korean Pig Farm. Journal of Inflammatory and Infectious Medicine 2025, 1 (3), 2. https://doi.org/10.53941/jiim.2025.100014.
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