Vernonia cinerea Extract and Its Silver Nanoparticles in the Making of Indigenous Eyeliner: A Formulation and Evaluation Study

Priya Prasannakumar; Sruthi Manikandan; Babitha T. Dharmapalan; Robert A. Antony

doi:10.53941/jmnp.2026.100015

Abstract

Background, Vernonia cinerea Less. (Asteraceae), known as “Poovaamkurunnel” in Malayalam and “Sahadevi” in Sanskrit, is a medicinally significant herb documented in Ayurvedic and other traditional medicine systems for its anti-inflammatory, analgesic, antipyretic, and antioxidant activities, with specific ethnomedicinal application in eye care, including conjunctivitis and as an anti-cataract agent. The present study aimed at using green synthesis for making silver nanoparticles (AgNPs) with the aqueous extract of Vernonia cinerea (Vc) to make VcAgNPs (Vernonia cinerea extract silver nanoparticles), and to incorporate these into an eyeliner formulation prepared by the indigenous burning method. VcAgNPs were characterized by standard physicochemical techniques. Antimicrobial activity of the extracts and finished products was evaluated against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538P. The carbon soot obtained was additionally tested against these organisms and Candida albicans ATCC 2091. Microbial safety of the formulations was also assessed. Physical stability studies confirmed that all three eyeliner formulations maintained their properties over six months. The major finding was that although both the Vc and VcAgNPs fresh extracts demonstrated notable antimicrobial activity, the finished eyeliners showed no inhibitory activity against the tested organisms, consistent with the expected degradation of bioactive compounds during the high-temperature burning process. Conclusion, The formulated eyeliners were physically and microbiologically safe over the study period, though they did not retain measurable antimicrobial activity after processing. These preliminary findings suggest the traditional preparation method may compromise the extract’s bioactive properties, and further studies are needed to evaluate the formulation’s broader safety and any potential ophthalmic therapeutic benefit.

References

1.
Amicone, S.; Solard, B.; Amelirad, S.; et al. Eye makeup in Northwestern Iran at the time of the Assyrian Empire: A new kohl recipe based on manganese and graphite from Kani Koter (Iron Age III). Archaeometry 2026, 68, S7–S21. https://doi.org/10.1111/arcm.13097.
2.
Ali, M.A.; Khalid, M.; Hamiduddin; et al. History of Ilmul Saidala (Unani Pharmacy) Through Ages: A Critical Appraisal and Current Scenario. Bangladesh J. Med. Sci. 2022, 21, 24–36. https://doi.org/10.3329/bjms.v21i1.56324.
3.
Waghulde, S.; Pawar, A.; Kadav, M.; et al. Formulation Development of a Patient Friendly Dosage Form for Eye Drug Delivery: Kajal. Proceedings 2019, 9, 69. https://doi.org/10.3390/ecsoc-22-05663.
4.
Dharkar, E.P. Tracing the Genesis of Kajalshalaka (Kohl Stick). Herit. J. Multidiscip. Stud. Archaeol. 2024, 11, 1157–1168.
5.
Sukanya, K.; Sujathamma, K. Netra Raksha: Safeguarding vision through Ayurvedic wisdom. J. Ayurveda Integr. Med. Sci. 2025, 10, 62–68. https://doi.org/10.21760/jaims.10.8.12.
6.
Surangi, K.G.; Fiaz, S.; Kumar, S.P. Review of Anjana (Corrylium) Procedure and Its Probable Mode of Action. Int. J. Ayurveda Pharma Res. 2016, 4, 34–42. Available online: https://ijapr.in/index.php/ijapr/article/view/369 (accessed on 23 March 2026).
7.
Dogra, N.K.; Kumar, S. A review on ethno-medicinal uses and pharmacology of Vernonia cinerea Less. Nat. Prod. Res. 2015, 29, 1102–1117. https://doi.org/10.1080/14786419.2014.981814.
8.
Trang, N.M.; Vinh, L.B.; Phong, N.V.; et al. Traditional Uses, Phytochemistry, and Pharmacological Activities of Vernonia cinerea (L.) Less.: An Updated Review. Nutrients 2024, 16, 1396. https://doi.org/10.3390/nu16091396.
9.
Haque, M.A.; Hassan, M.M.; Das, A.; et al. Phytochemical investigation of Vernonia cinerea (Family: Asteraceae). J. Appl. Pharm. Sci. 2012, 2, 79–83. https://doi.org/10.7324/JAPS.2012.2617.
10.
Vimala; Hans, D. Leaf Epidermal Features and Ethnomedicinal Studies of Vernonia cinerea (L.) Less. from Semi-arid Regions of Barmer, Rajasthan, India. J. Plant Sci. Res. 2023, 39, 31–38. https://doi.org/10.32381/JPSR.2023.39.03.17.
11.
Toyang, N.J.; Verpoorte, R. A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). J. Ethnopharmacol. 2013, 146, 681–723. https://doi.org/10.1016/j.jep.2013.01.040.
12.
Theja, D.D.; Nirmala, S. A review of Vernonia cinerea L. ethno-medicinal uses and pharmacology shows that it could be a useful plant for medical purposes. Intell. Pharm. 2024, 2, 662–671. https://doi.org/10.1016/j.ipha.2023.11.005.
13.
Alara, O.R.; Abdurahman, N.H.; Mudalip, S.K.A.; et al. Phytochemical and pharmacological properties of Vernonia amygdalina: A review. J. Chem. Eng. Ind. Biotechnol. 2017, 2, 80–96. https://doi.org/10.15282/jceib.v2i1.3871.
14.
Lai, G.-R.; Wu, C.-S. Analyzing of Major Active Luteolin in Vernonia cinerea. Int. J. Biosci. Biochem. Bioinform. 2013, 3, 363–367. https://doi.org/10.7763/IJBBB.2013.V3.233.
15.
Adesanoye, O.A.; Farombi, E.O. Hepatoprotective effects of Vernonia amygdalina (Astereaceae) in rats treated with carbon tetrachloride. Exp. Toxicol. Pathol. 2010, 62, 197–206. https://doi.org/10.1016/j.etp.2009.05.008.
16.
Sonibare, M.A.; Aremu, O.T.; Okorie, P.N. Antioxidant and antimicrobial activities of solvent fractions of Vernonia cinerea (L.) Less leaf extract. Afr. Health Sci. 2016, 16, 629–639. https://doi.org/10.4314/ahs.v16i2.34.
17.
Asha, R.; Gayathri Devi, V.; Abraham, A. Lupeol, a pentacyclic triterpenoid isolated from Vernonia cinerea attenuate selenite induced cataract formation in Sprague Dawley rat pups. Chem. Biol. Interact. 2016, 245, 20–29. https://doi.org/10.1016/j.cbi.2015.12.002.
18.
Mishra, N.; Gupta, E.; Singh, P.; et al. Insight on Vernonia Plant for Its Pharmacological Properties: A review. Recent Adv. Food Nutr. Agric. 2023, 14, 84–93. https://doi.org/10.2174/2212798412666230330164954.
19.
Karbassi, E.; Amiri-Ardekani, E.; Farsinezhad, A.; et al. The Efficacy of Kohl (Surma) and Erythromycin in Treatment of Blepharitis: An Open-Label Clinical Trial. Evid. Based Complement. Altern. Med. 2022, 2022, 6235857. https://doi.org/10.1155/2022/6235857.
20.
Ramaswamy, U.; Mukundan, D.; Sreekumar, A.; et al. Green Synthesis and Characterization of Silver Nanoparticles Using Aqueous Whole Plant Extract of Vernonia cinerea L. and its Biological Activities. Mater. Today Proc. 2015, 2, 4600–4608. https://doi.org/10.1016/j.matpr.2015.10.080.
21.
Stozhko, N.; Tarasov, A.; Tamoshenko, V.; et al. Green Silver Nanoparticles: Plant-Extract-Mediated Synthesis, Optical and Electrochemical Properties. Physchem 2024, 4, 402–419. https://doi.org/10.3390/physchem4040028.
22.
Majani, S.S.; Ramesh, K.B.; Devendra, B.K.; et al. Bio-combustion synthesis of silver nanoparticles and evaluation of their effective antibacterial and photodegradation properties. Sci. Rep. 2026, 16, 2316. https://doi.org/10.1038/s41598-025-32173-w.
23.
Amendola, V.; Meneghetti, M. Size Evaluation of Gold Nanoparticles by UV−vis Spectroscopy. J. Phys. Chem. C 2009, 113, 4277–4285. https://doi.org/10.1021/jp8082425.
24.
Zani, M.; Ahmad, H.; Razak, A. Synthesis and characterisation of silver nanoparticles using Vernonia cinerea aqueous extract and their cytotoxicity activity against Kasumi-1 cell line. J. Teknol. 2016, 78, 57–65. https://doi.org/10.11113/jt.v78.9873.
25.
Srinivasan, M.; Chandane, A.Y.; Tagalpallewar, A.A.; et al. Kerala’s anticancer flora: A comprehensive review. J. Appl. Pharm. Sci. 2024, 14, 018–045. https://doi.org/10.7324/JAPS.2024.180248.
26.
Bhandari, S.; Roy, A.; Ali, M.S.; et al. Cotton soot derived carbon nanoparticles for NiO supported processing temperature tuned ambient perovskite solar cells. Sci. Rep. 2021, 11, 23388. https://doi.org/10.1038/s41598-021-02796-w.
27.
Ministry of Health and Family Welfare, Government of India. The Drugs and Cosmetics Act and Rules the Drugs and Cosmetics Act. 1940. Available online: https://cdsco.gov.in/opencms/export/sites/CDSCO_WEB/Pdf-documents/acts_rules/2016DrugsandCosmeticsAct1940Rules1945.pdf (accessed on 14 August 2026).
28.
ISO 17516:2014; Cosmetics—Microbiology—Microbiological Limits. ISO: Geneva, Switzerland, 2014. Available online: https://www.iso.org/standard/59938.html (accessed on 23 March 2026).
29.
Dibrov, P.; Dzioba, J.; Gosink, K.K.; et al. Chemiosmotic mechanism of antimicrobial activity of Ag(+) in Vibrio cholerae. Antimicrob. Agents Chemother. 2002, 46, 2668–2670. https://doi.org/10.1128/AAC.46.8.2668-2670.2002.
30.
Sahayaraj, K.; Roobadevi, M.; Rajesh, S.; et al. Vernonia cinerea (L.) Less. silver nanocomposite and its antibacterial activity against a cotton pathogen. Res. Chem. Intermed. 2015, 41, 5495–5507. https://doi.org/10.1007/s11164-014-1676-8.
31.
Randive, D.S.; Bhinge, S.D.; Jadhav, N.R.; et al. Assessment of antimicrobial efficacy of kohl/kajal prepared by different Indian methods against selected microbial strains. Int. J. Curr. Pharm. Sci. 2020, 12, 37–44. https://doi.org/10.22159/ijcpr.2020v12i3.38302.
32.
Dayrit, F.M. The Properties of Lauric Acid and Their Significance in Coconut Oil. J. Am. Oil Chem. Soc. 2015, 92, 1–15. https://doi.org/10.1007/s11746-014-2562-7.
33.
Paramelle, D.; Sadovoy, A.; Gorelik, S.; et al. A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra. Analyst 2014, 139, 4855–4861. https://doi.org/10.1039/C4AN00978A.
34.
Iravani, S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011, 13, 2638–2650. https://doi.org/10.1039/C1GC15386B.
35.
Mittal, A.K.; Chisti, Y.; Banerjee, U.C. Synthesis of metallic nanoparticles using plant extracts. Biotechnol. Adv. 2013, 31, 346–356. https://doi.org/10.1016/j.biotechadv.2013.01.003.
36.
Agnihotri, S.; Mukherji, S.; Mukherji, S. Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy. RSC Adv. 2014, 4, 3974–3983. https://doi.org/10.1039/C3RA44507K.
37.
Kim, J.S.; Kuk, E.; Yu, K.N.; et al. Antimicrobial effects of silver nanoparticles. Nanomedicine 2007, 3, 95–101. https://doi.org/10.1016/j.nano.2006.12.001.
38.
Latha, L.Y.; Darah, I.; Jain, K.; et al. Toxicity study of Vernonia cinerea. Pharm. Biol. 2010, 48, 101–104. https://doi.org/10.3109/13880200903046203.
39.
Mirghaffari, M.; Rezaei Sedehi, H.; Mahboubizadeh, S.; et al. Carbon Quantum Dots as Next-Generation Nanocarriers for Targeted Ocular Drug Delivery: Innovations and Therapeutic Potential—A Review. Prog. Chem. Biochem. Res. 2025, 8, 208–233. https://doi.org/10.48309/pcbr.2025.505208.1393.

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