Influence of Germination on Physicochemical, Mineral, Textural, and Nutritional Properties of Sorghum and Its Application in Gluten-Free Health Bar Development

Mehak Katyal; Sheetal Thakur; Aparna Kumari; Arun Kumar; Saloni Joshi

doi:10.53941/fm.2026.100006

Abstract

The present study aimed to evaluate the nutritional enhancement of sorghum through germination and its application in gluten-free health bar development. Sorghum grains were germinated for 48 h, dried at 60 °C for 7 h 35 min, and milled into germinated sorghum flour (GSF), while un-germinated sorghum flour (UGSF) was prepared similarly without sprouting. The different formulations of health bars were developed: control (no sorghum), 25% UGSF, and 25% GSF. Proximate analysis revealed that germination significantly increased the protein content of the flour from 6.83% in UGSF to 13.87% in GSF, while the crude fiber content increased from 2.32% to 10% in GSF. The textural analysis indicated that GSF bars had lower hardness (53.71 N) compared to UGSF bars (212.52 N), with reduced gumminess and chewiness, suggesting a softer, more palatable product. The study demonstrated that germination of sorghum effectively enhances nutritional and functional properties, and its incorporation into gluten-free health bars can provide a protein- and fiber-rich functional snack suitable for health-conscious and gluten-sensitive populations.

References

1.
Adeyeye, E.I.; Adesina, A.J.; Olaleye, A.A. Chemical and functional evaluations of raw, steeped and germinated sorghum bicolor. Anal. Chem. Indian J. 2018, 19, 147–155.
2.
Afify, A.E.M.M.; El-Beltagi, H.S.; Abd El-Salam, S.M.; et al. Bioavailability of iron, zinc, phytate and phytase activity during soaking and germination of white sorghum varieties. PLoS ONE 2011, 6, e25512.
3.
Cheryan, M.; Rackis, J.J. Phytic acid interactions in food systems. Crit. Rev. Food Sci. Nutr. 1980, 13, 297–335.
4.
Oatway, L.; Vasanthan, T.; Helm, J.H. Phytic acid. Food Rev. Int. 2001, 17, 419–431.
5.
Agrahar-Murugkar, D.; Zaidi, A.; Dwivedi, S. Development of gluten free eggless cake using gluten free composite flours made from sprouted and malted ingredients and its physical, nutritional, textural, rheological and sensory properties evaluation. J. Food Sci. Technol. 2018, 55, 2621–2630.
6.
Bagarinao, N.C.; King, J.; Leong, S.Y.; et al. Effect of germination on seed protein quality and secondary metabolites and potential modulation by pulsed electric field treatment. Foods 2024, 13, 1598. https://doi.org/10.3390/foods13111598.
7.
Borgonovi, S.M.; Marzocchi, S.; Pasini, F.; et al. Exploring Germination to Unlock the Nutritional Potential of Sorghum (Sorghum bicolor). Molecules 2025, 30, 3622.
8.
Gunathunga, C.; Senanayake, S.; Jayasinghe, M.A.; et al. Germination effects on nutritional quality: A comprehensive review of selected cereals and pulses changes. J. Food Compos. Anal. 2024, 128, 106024.
9.
Elkhalifa, A.E.O.; Bernhardt, R.; Cardone, G.; et al. Physicochemical properties of sorghum flour are selectively modified by combined germination-fermentation. J. Food Sci. Technol. 2017, 54, 3307–3313.
10.
Singh, A.K.; Rehal, J.; Kaur, A.; et al. Enhancement of attributes of cereals by germination and fermentation: A review. Crit. Rev. Food Sci. Nutr. 2015, 55, 1575–1589. https://doi.org/10.1080/10408398.2012.706661.
11.
Ali, A.S.; Elozeiri, A.A. Metabolic processes during seed germination. Adv. Seed Biol. 2017, 2017, 141–166.
12.
Kayisoglu, C.; Altikardes, E.; Guzel, N.; et al. Germination: A powerful way to improve the nutritional, functional, and molecular properties of white-and red-colored sorghum grains. Foods 2024, 13, 662.
13.
Klerks, M.; Román, S.; Verkerk, R.; et al. Are cereal bars significantly healthier and more natural than chocolate bars? A preliminary assessment in the German market. J. Funct. Foods 2022, 89, 104940.
14.
Nath, A.; Dutta, D.; Kumar, P.; et al. Review on recent advances in value addition of jaggery based products. J. Food Process Technol. 2015, 6, 1000440.
15.
Cena, H.; Calder, P.C. Defining a Healthy Diet: Evidence for The Role of Contemporary Dietary Patterns in Health and Disease. Nutrients 2020, 12, 334. https://doi.org/10.3390/nu12020334.
16.
Tasie, M.M.; Gebreyes, B.G. Characterization of nutritional, antinutritional, and mineral contents of thirty-five sorghum varieties grown in Ethiopia. Int. J. Food Sci. 2020, 2020, 8243617.
17.
AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists, 15th ed.; Association of Official Analytical Chemists: Rockville, MD, USA, 1990.
18.
Bharadhan, K.G.; Janani, M.; Abishek, K.S. Innovations in energy bars: Formulation, nutritional analysis, and consumer perspectives. Int. J. Manag. Res. Bus. Strategy 2024, 13, 1–13.
19.
Eid, W.A.; Azab, D.E.S.H.; Negm, S.H. Characterization of a novel date energy bar fortified with Moringa oleifera leaves powder. J. Future Foods 2025, 5, 266–275.
20.
Bhinder, S.; Kaur, A.; Singh, B.; et al. Proximate composition, amino acid profile, pasting and process characteristics of flour from different Tartary buckwheat varieties. Food Res. Int. 2020, 130, 108946.
21.
Kumar, A.; Singh, N. Embracing nutritional, physical, pasting, textural, sensory and phenolic profile of functional muffins prepared by partial incorporation of lyophilized wheatgrass, fenugreek and basil microgreens juice powder. J. Sci. Food Agric. 2024, 104, 4286–4295. https://doi.org/10.1002/jsfa.13314.
22.
Lu, T.M.; Lee, C.C.; Mau, J.L.; et al. Quality and antioxidant property of green tea sponge cake. Food Chem. 2010, 119, 1090–1095.
23.
Khatoon, N.; Prakash, J. Nutrient retention in microwave cooked germinated legumes. Food Chem. 2006, 97, 115–121.
24.
Marchini, M.; Marti, A.; Folli, C.; et al. Sprouting of sorghum (Sorghum bicolor [L.] Moench): Effect of drying treatment on protein and starch features. Foods 2021, 10, 407.
25.
Chandra, S.; Singh, S.; Kumari, D. Evaluation of functional properties of composite flours and sensorial attributes of composite flour biscuits. J. Food Sci. Technol. 2015, 52, 3681–3688.
26.
Tizazu, S.; Urga, K.; Abuye, C.; et al. Improvement of energy and nutrient density of sorghum-based complementary foods using germination. Afr. J. Food Agric. Nutr. Dev. 2010, 10, 2927–2942.
27.
Afify, A.E.M.M.; El-Beltagi, H.S.; Abd El-Salam, S.M.; et al. Protein solubility, digestibility and fractionation after germination of sorghum varieties. PLoS ONE 2012, 7, e31154. https://doi.org/10.1371/journal.pone.0031154.
28.
Krishnan, V.; Awana, M.; Singh, A.; et al. Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum). Int. J. Biol. Macromol. 2021, 183, 1087–1095.
29.
Cardone, G.; Rumler, R.; Speranza, S.; et al. Sprouting time affects sorghum (Sorghum bicolor [L.] Moench) functionality and bread-baking performance. Foods 2021, 10, 2285.
30.
Kokani, R.C.; Bharat, H.P.; Kelgane, S. Studies on utilization of Ragi for preparation of malted ragi cookies. Int. J. Sci. Res. 2018, 7, 28–32.
31.
Kristanti, D.; Setiaboma, W.; Iwansyah, A.C.; et al. Study on red and white sorghum (Sorghum bicolor) germination flour: Physicochemical properties and it’s correlation. BIO Web Conf. 2023, 69, 03007.
32.
Singh, A.; Kumari, A.; Chauhan, A.K. Formulation and evaluation of novel functional snack bar with amaranth, rolled oat, and unripened banana peel powder. J. Food Sci. Technol. 2022, 59, 3511–3521.
33.
Trappey, E.F.; Khouryieh, H.; Aramouni, F.; et al. Effect of sorghum flour composition and particle size on quality properties of gluten-free bread. Food Sci. Technol. Int. 2015, 21, 188–202. https://doi.org/10.1177/1082013214523632.
34.
Zhao, R.; Ghazzawi, N.; Wu, J.; et al. Germinated brown rice attenuates atherosclerosis and vascular inflammation in low-density lipoprotein receptor-knockout mice. J. Agric. Food Chem. 2018, 66, 4512–4520.
35.
Ikram, A.; Saeed, F.; Afzaal, M.; et al. Nutritional and end-use perspectives of sprouted grains: A comprehensive review. Food Sci. Nutr. 2021, 9, 4617–4628. https://doi.org/10.1002/fsn3.2408.
36.
Krishnan, R.; Sakhare, S.D.; Meera, M.S. Effect of germination-hydrothermal treatments followed by fractionation on inhibitory factors, β-carotene and iron-zinc bioaccessibility in pearl millet fractions. J. Cereal Sci. 2020, 93, 102930.
37.
Elliott, H.; Woods, P.; Green, B.D.; et al. Can sprouting reduce phytate and improve the nutritional composition and nutrient bioaccessibility in cereals and legumes? Nut. Bull. 2022, 47, 138–156.
38.
Verma, S.; Khetrapaul, N.; Verma, V. Development & standardization of iron rich sorghum based cereal bars and their nutrient composition. J. Pharmacogn. Phytochem. 2018, 7, 1189–1192.
39.
Pelembe, L.A.M.; Dewar, J.; Taylor, J.R.N. Food products from malted pearl millet. In Proceeding of the Workshop on the Proteins of Sorghum and Millets: Enhancing Nutritional and Functional Properties for Africa, Pretoria, South Africa, 2–4 April 2003.
40.
Okpala, L.; Ekwe, O.O. Nutritional quality of cookies produced from mixtures of fermented pigeon pea, germinated sorghum and cocoyam flours. Eur. J. Food Res. Rev. 2013, 3, 38–49.
41.
Loveday, S.M.; Hindmarsh, J.P.; Creamer, L.K.; et al. Physicochemical changes in a model protein bar during storage. Food Res. Int. 2009, 42, 798–806.
42.
Selokar, N.; Vidyalakshmi, R.; Thiviya, P.; et al. Assessment of nutritional quality of non-conventional millet malt enriched bar. J. Food Process. Preserv. 2022, 46, e17271.
43.
Safvi, A.F.; Ahmad, A.; Younis, K.; et al. Development of energy bar by adding underutilized Chironji (Buchanania lanzan) seeds. Food Humanit. 2023, 1, 44–50.
44.
Małecki, J.; Tomasevic, I.; Djekic, I.; et al. The effect of protein source on the physicochemical, nutritional properties and microstructure of high-protein bars intended for physically active people. Foods 2020, 9, 1467.
45.
Momanyi, D.; Owino, W.; Makokha, A. Formulation, nutritional and sensory evaluation of baobab based ready-to-eat sorghum and cowpea blend snack bars. Sci. Afr. 2020, 7, e00215.
46.
Mantadakis, E.; Chatzimichael, E.; Zikidou, P. Iron deficiency anemia in children residing in high and low-income countries: Risk factors, prevention, diagnosis and therapy. Mediterr. J. Hematol. Infect. Dis. 2020, 12, e2020041.
47.
Liu, L.; Jiang, X.; Chen, Y.; et al. Germination-induced modifications of starch structure, flour-processing characteristics, and in vitro digestive properties in maize. Food Chem. X 2024, 22, 101430.
48.
Ocheme, O.B.; Adedeji, O.E.; Lawal, G.; et al. Effect of germination on functional properties and degree of starch gelatinization of sorghum flour. J. Food Res. 2015, 4, 159.
49.
Uppal, V.; Bains, K. Effect of germination periods and hydrothermal treatments on in vitro protein and starch digestibility of germinated legumes. J. Food Sci. Technol. 2012, 49, 184–191. https://doi.org/10.1007/s13197-011-0273-8.

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