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2507000979
  • Open Access
  • Review
Cas9 Cis/Trans Cleavage Mechanisms: Cross-System CRISPR Insights and Diagnostic Applications
  • Xueying Li,   
  • Ying Chen,   
  • Linglong Huang,   
  • Jiyun Chen *,   
  • Liang Liu *

Received: 17 Mar 2025 | Revised: 11 Apr 2025 | Accepted: 15 May 2025 | Published: 16 Jul 2025

Abstract

CRISPR-Cas system is an adaptive immune system of prokaryotes against foreign invading viruses. Type II CRISPR-Cas9 system, as the earliest of the Class II systems to be discovered and applied to gene editing, has been recently found to possess trans-cleavage activity with other Class II systems not long ago. In this review, we summarize the molecular mechanism of cis- and trans-cleavage of target nucleic acids by the Class II type II CRISPR-Cas system, introduce the nucleic acid detection platforms developed based on its trans-cleavage activity, and compare them with those developed based on the type V, type VI, and type I CRISPR-Cas systems.

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DOI
10.53941/hm.2025.100021
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Volume 2, Issue 3
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Li, X.; Chen, Y.; Huang, L.; Chen, J.; Liu, L. Cas9 Cis/Trans Cleavage Mechanisms: Cross-System CRISPR Insights and Diagnostic Applications. Health and Metabolism 2025, 2 (3), 6. https://doi.org/10.53941/hm.2025.100021.
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